BIO 207 CBT PRACTICE

1. Specify the culture type that involves the growth of individual plant cells in an agitated liquid medium.

2. Highlight the main drawback of virus-mediated transfection in clinical research.

3. Identify the key advantage of RNA interference in clinical applications

4. Determine the type of animal cell culture that originates directly from tissue.

5. Specify the term for cell lines capable of infinite growth.

6. Specify the type of tissue culture that involves maintaining an entire organ.

7. Identify the organelle targeted in electroporation to deliver genetic material.

8. Define the main purpose of transfection in biological research

9. Identify the growth phase where roots are formed during plant tissue culture.

10. Determine the primary disadvantage of virus-mediated transfection.

11. State the importance of aseptic techniques in cell culture laboratories

12. Identify the equipment used to count cells in suspension.

13. Determine the major risk associated with using serum in cell culture.

14. Highlight the transfection method that uses a short laser pulse to create transient pores in membranes.

15. Specify the chemical method most widely used for transfection.

16. Determine the primary use of an inverted phase-contrast microscope in cell culture.

17. Determine the culture type that involves growing isolated single cells.

18. Specify the technique used to detach strongly adherent cells during subculture

19. Identify the plant culture method that uses pollen grains for haploid plant generation.

20. Specify the role of phenol red in cell culture media.

21. Highlight the disadvantage of continuous cell lines in biological research.

22. Identify the transfection method that integrates genetic material into the host genome.

23. State the term for using enzymes to harvest cells during subculturing.

24. Name the proteolytic enzyme commonly used to detach adherent cells during subculture.

25. Identify the type of transfection that does not integrate genetic material into the host genome.

26. Determine the main drawback of serum in cell culture media.

27. Identify the chemical used to adjust the pH of plant tissue culture media

28. Highlight a key advantage of mRNA transfection over DNA transfection.

29. State the role of sodium hydroxide in preparing plant tissue culture media.

30. Identify the organism commonly used for viral vector transfection in humans.

31. Highlight the main advantage of primary cell cultures.

32. Highlight the advantage of using serum-free media in cell culture.

33. Name the component of cell culture media that ensures physiological pH is maintained.

34. Highlight the key function of EDTA in cell harvesting.

35. Highlight the main function of laminar flow hoods in cell culture laboratories.

36. ‍State the purpose of subculturing cells in a culture system.

37. Specify the method used to ensure sterility of culture media.

38. Identify the physical transfection method that uses electric pulses to permeabilize cell membranes.

39. Highlight the culture type used for maintaining an unorganized mass of proliferative plant cells.

40. Specify the physical transfection method that uses gold particles to deliver genetic material.