BIO 207 CBT PRACTICE

1. Determine the major risk associated with using serum in cell culture.

2. ‍State the purpose of subculturing cells in a culture system.

3. Determine the type of animal cell culture that originates directly from tissue.

4. Highlight the main function of laminar flow hoods in cell culture laboratories.

5. Specify the type of tissue culture that involves maintaining an entire organ.

6. Specify the term for cell lines capable of infinite growth.

7. Determine the primary disadvantage of virus-mediated transfection.

8. Identify the key advantage of RNA interference in clinical applications

9. Identify the transfection method that integrates genetic material into the host genome.

10. Identify the plant culture method that uses pollen grains for haploid plant generation.

11. Specify the chemical method most widely used for transfection.

12. Specify the technique used to detach strongly adherent cells during subculture

13. Determine the primary use of an inverted phase-contrast microscope in cell culture.

14. State the importance of aseptic techniques in cell culture laboratories

15. Identify the type of transfection that does not integrate genetic material into the host genome.

16. Determine the main drawback of serum in cell culture media.

17. Determine the culture type that involves growing isolated single cells.

18. Specify the physical transfection method that uses gold particles to deliver genetic material.

19. Identify the organism commonly used for viral vector transfection in humans.

20. Identify the equipment used to count cells in suspension.

21. Highlight a key advantage of mRNA transfection over DNA transfection.

22. Specify the role of phenol red in cell culture media.

23. Highlight the advantage of using serum-free media in cell culture.

24. Specify the culture type that involves the growth of individual plant cells in an agitated liquid medium.

25. Highlight the disadvantage of continuous cell lines in biological research.

26. Highlight the culture type used for maintaining an unorganized mass of proliferative plant cells.

27. Identify the organelle targeted in electroporation to deliver genetic material.

28. Identify the growth phase where roots are formed during plant tissue culture.

29. State the term for using enzymes to harvest cells during subculturing.

30. Highlight the transfection method that uses a short laser pulse to create transient pores in membranes.

31. Highlight the main advantage of primary cell cultures.

32. State the role of sodium hydroxide in preparing plant tissue culture media.

33. Identify the chemical used to adjust the pH of plant tissue culture media

34. Name the component of cell culture media that ensures physiological pH is maintained.

35. Specify the method used to ensure sterility of culture media.

36. Highlight the main drawback of virus-mediated transfection in clinical research.

37. Define the main purpose of transfection in biological research

38. Highlight the key function of EDTA in cell harvesting.

39. Name the proteolytic enzyme commonly used to detach adherent cells during subculture.

40. Identify the physical transfection method that uses electric pulses to permeabilize cell membranes.