BIO 207 CBT PRACTICE

1. Highlight the disadvantage of continuous cell lines in biological research.

2. Highlight the key function of EDTA in cell harvesting.

3. State the term for using enzymes to harvest cells during subculturing.

4. Determine the primary disadvantage of virus-mediated transfection.

5. ‍State the purpose of subculturing cells in a culture system.

6. Identify the key advantage of RNA interference in clinical applications

7. Specify the technique used to detach strongly adherent cells during subculture

8. Name the component of cell culture media that ensures physiological pH is maintained.

9. Identify the type of transfection that does not integrate genetic material into the host genome.

10. Specify the term for cell lines capable of infinite growth.

11. Highlight the transfection method that uses a short laser pulse to create transient pores in membranes.

12. Identify the physical transfection method that uses electric pulses to permeabilize cell membranes.

13. Specify the role of phenol red in cell culture media.

14. Highlight the advantage of using serum-free media in cell culture.

15. State the importance of aseptic techniques in cell culture laboratories

16. Determine the main drawback of serum in cell culture media.

17. Highlight the main drawback of virus-mediated transfection in clinical research.

18. Determine the primary use of an inverted phase-contrast microscope in cell culture.

19. Identify the organism commonly used for viral vector transfection in humans.

20. Specify the physical transfection method that uses gold particles to deliver genetic material.

21. Define the main purpose of transfection in biological research

22. State the role of sodium hydroxide in preparing plant tissue culture media.

23. Identify the organelle targeted in electroporation to deliver genetic material.

24. Determine the type of animal cell culture that originates directly from tissue.

25. Highlight the main advantage of primary cell cultures.

26. Specify the type of tissue culture that involves maintaining an entire organ.

27. Determine the major risk associated with using serum in cell culture.

28. Specify the culture type that involves the growth of individual plant cells in an agitated liquid medium.

29. Specify the method used to ensure sterility of culture media.

30. Identify the plant culture method that uses pollen grains for haploid plant generation.

31. Identify the chemical used to adjust the pH of plant tissue culture media

32. Highlight a key advantage of mRNA transfection over DNA transfection.

33. Highlight the main function of laminar flow hoods in cell culture laboratories.

34. Determine the culture type that involves growing isolated single cells.

35. Highlight the culture type used for maintaining an unorganized mass of proliferative plant cells.

36. Identify the transfection method that integrates genetic material into the host genome.

37. Identify the growth phase where roots are formed during plant tissue culture.

38. Identify the equipment used to count cells in suspension.

39. Specify the chemical method most widely used for transfection.

40. Name the proteolytic enzyme commonly used to detach adherent cells during subculture.